_theProject
Big model | selfstanding
Final model | fabrication
Final model | simulation
Hemp simulation 02 | explorations
Big model, 350mm high _ 01
Hemp simulation
Hemp structure – Gradient
Hemp structure – Walls
Tests aim to explore system behaviours on a different hemp arrangement. Two kinds of “walls” follow the hemp bending.
Sequential operations
- design and lasercut the plexi structure: plexiglass, diameter 95mm, thickness 2mm (thanks to MakeInBo)
- build hemp structures: distance between plates 85/50mm
- put the structure in a glass jar with some ml of water
- autoclave, 121°C – 20′
- (bio)plastic #2.1.2 spread on fibers
- inoculate with POS1 – Pleurotus Ostreatus
- growth in a dark room at 22.5°C for 18 days
Sample: #3.2.1 _ Simmetric Wall
Sample: #3.2.2 _ aSimmetric Wall
bio-analogic computation
Mycelium is a living agent-system. The hemp fiber structure built here connects two plexiglass plates and It provides a good substrate to mycelium growth. Result shows a redundant and hyper connected system: a fibrous composite material made of (and by) mycelium.
Sequential operations
- design and lasercut plates: plexiglass, diameter 75/88 mm, thickness 3mm (thanks to MakeInBo)
- build hemp structures: distance between plates 15cm
- put the structure in a glass jar with some ml of water
- autoclave, 121°C – 20′
- (bio)plastic #2.1.2 spread on fibers
- inoculate with POS1 – Pleurotus Ostreatus (5 infected wheat grains)
- growth in a dark room at 22.5°C for 12 days > image
Sample #3.1.1 – bio-analogic computation _ mycelium tectonics
Hemp structure – benchmark
After latest and satisfying inVitro results (1, 2), some simple structures eneble the possibility to investigate the 3th dimension. These “benchmark” are a kind of analogic computation to understend and finally explore mycelium tectonics, architecturally speaking.
Sequential operations
- design and lasercut the wood structure: poplar, diameter 60mm, thickness 4mm (thanks to MakeInBo)
- build hemp structures: distance between plates 10cm
- 24h in water (this step involved fiber bending and volume reduction)
- autoclave, 121°C – 20′
- (bio)plastic #2.1.2 spread on fibers > image
- inoculate with POS1 – Pleurotus Ostreatus (one infected wheat grain)
- put the structure in a glass jar with some ml of water to ensure humidity > image
- growth in a dark room at 22.5°C for 16 days > image
#3.0.1B – “gravity”
#3.0.2B – “double plate linear”
#3.0.3B – “double plate open”
#3.0.4B – “double plate bend&torsion”
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Pleurotus & Fiber & (bio)Plastic
A test to explore mycelium behaviours on a substrate made by hemp fiber and (bio)Plastic. Once Pleurotus is sufficiently grown, petri capsula was opened to let it air-dry.
Results show an high tensile strength fibrous composite.
(bio)plastic recipe: #2.1
mycelium: POS 1 _ Pleurotus Ostreatus
Pleurotus & Fiber
(bio)plastic benchmark
These tests aim to understand if starch plastic could be a good substrate for micelia. After a lot of experiments, finally I found the plastic that has the right consistency to be spread on fiber.
(bio)plastic recipe: #2.1
85% H20
6% Starch
4% Carboxymethyl cellulose sodium salt
3% Glycerol
2% PDA – potato dextrose agar
0.1% Ammonium Acetate
Micelia used for the test:
POS 1 _ pleurotus ostreatus
GLU 2 _ ganoderma lucidum
GLU 16 _ ganoderma lucidum
Recipe #2.1 after 4 days and 8 days:
in vitro petriMask
Exploring boundaries
How does the mycelium explore boundaries and build bridge connetions from landscape morphology?
This test allows to understand mycelium’s behaviours of growth through PLA substrate.
To do that a 3d printed mask was insert into a petri dish before the sterilization.
Substrates are:
LIGN _ Lignosulphonate bretax C 1,5% + AA 1% | pH: 6,12
CELL _ carboxymethyl cellulose sodium salt 1,5% + AA 1% | pH: 5,92
Micelia are:
GLU2 _ ganoderma lucidum (inoculated on 18th of September 2014)
POS1 _ pleurotus ostreatus (inoculated on 18th of September 2014)
ARM _ armillaria mellea (inoculated on 18th of September 2014)
Thanks to MakeInBo
Ganoderma Lucidum after 6 days, 9 days and 21 days:
in vitro mycoTests 02
Previous in vitro mycoTests here.
Lignin and cellulose substrate
Some tests highlight behavioral growth differences of three species of micelia. Substrates were prepared and filled in petri capsules divided in three sub-compartments.
Substrates are:
AA _ agar agar grade A | pH: 6,038 (test)
LIGN _ Lignosulphonate bretax C 1,5% + AA 1% | pH: 6,12
CELL _ carboxymethyl cellulose sodium salt 1,5% + AA 1% | pH: 5,92
Micelia are:
ARM _ armillaria mellea (inoculated on 18th of September 2014)
GLU2 _ ganoderma lucidum (inoculated on 18th of September 2014)
PCO1 _ pleurotus cornucopie (inoculated on 18th of September 2014)
Armillaria Mellea after 6 days, 9 days and 21 days:
in vitro mycoTests
Grazie alla disponibilità offertami dal Centro di Micologia Applicata della facoltà di Agraria dell’Università di Bologna, ho eseguito alcuni test con lo scopo di iniziare ad indagare la possibilità di guidare e indirizzare la crescita del micelio.
Ringrazio per il prezioso contributo la Dott.sa Federica Piattoni, la Prof.sa Alessandra Zambonelli e i Dott. Mirco Iotti e Dott.sa Pamela Leonardi per i consigli.
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The mycology centre of the Agriculture Faculty at the University of Bologna offered me the possibility to work at their Lab and make some in vitro tests with mycelia. The purpose is trying to guide and lead the mycelium growth in a controlled manner.
Thanks to Dott.sa Federica Piattoni, Prof.sa Alessandra Zambonelli, Dott. Mirco Iotti and Dott.sa Pamela Leonardi for their help and suggestions.
glucose handmade pattern
Un primo set di capsule petri è stato preparato lavorando su A-A (agar agar grade a) a diverse concetrazioni di zucchero (glucosio).
Continua a leggere
visualization test 02
A really glow night experience…
visualization test
Some exploration result made on a heterogenic substrate with tropism influance.
Renders made with blender. It’s a glossy steel material covered by mesh wireframe.
click to enlarge
mycelium tectonics, a processing code.
My code has reached version 1.0 and here I am to explain some new features and change log.
change.LOG:
1.0 > select folder to upload substrate space >> cpSel_Sub()
creation of run() and runSurface()
title at the beginning
agent_data no more > all agent information are stored in substrate_data/"subfolder"
export CSV > add "name" and "frameCount"
temporary info > sPrint: string to print & toPrint: boolean to activate print procedure
0.9 > export file image+mesh+parameters in a subfolder /export-data/YYYYMMDD
I found very usefull the possibility to choose different substrates at the beginning of the sketch, playing the simulation with some parameters and run again the sketch without changing them.
The controlP5 library and the class listBox let me the chance to look into a system folder and choose a different substrate at any time.
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