Hemp structure – benchmark

After latest and satisfying inVitro results (1, 2), some simple structures eneble the possibility to investigate the 3th dimension. These “benchmark” are a kind of analogic computation to understend and finally explore mycelium tectonics, architecturally speaking.

Sequential operations
  • design and lasercut the wood structure: poplar, diameter 60mm, thickness 4mm (thanks to MakeInBo)
  • build hemp structures: distance between plates 10cm
  • 24h in water (this step involved fiber bending and volume reduction)
  • autoclave, 121°C – 20′
  • (bio)plastic #2.1.2 spread on fibers > image
  • inoculate with POS1 – Pleurotus Ostreatus (one infected wheat grain)
  • put the structure in a glass jar with some ml of water to ensure humidity > image
  • growth in a dark room at 22.5°C for 16 days > image

#3.0.1B – “gravity”

sample_#3.0.1B_t0 sample_#3.0.1B_t1-2

sample_#3.0.1B_A sample_#3.0.1B_B
sample_#3.0.1B_E

#3.0.2B – “double plate linear”

sample_#3.0.2B_t0 sample_#3.0.2B_t1

sample_#3.0.2B_A sample_#3.0.2B_B

#3.0.3B – “double plate open”

sample_#3.0.3B_t0 sample_#3.0.3B_t1

sample_#3.0.3B_A sample_#3.0.3B_B-2
sample_#3.0.3B_C

#3.0.4B – “double plate bend&torsion”

sample_#3.0.4B_t0 sample_#3.0.4B_t1

in vitro mycoTests 02

Previous in vitro mycoTests here.

Lignin and cellulose substrate

Some tests highlight behavioral growth differences of three species of micelia. Substrates were prepared and filled in petri capsules divided in three sub-compartments.

Substrates are:
AA _ agar agar grade A | pH: 6,038 (test)
LIGN _ Lignosulphonate bretax C 1,5% + AA 1% | pH: 6,12
CELL _ carboxymethyl cellulose sodium salt 1,5% + AA 1% | pH: 5,92

Micelia are:
ARM _ armillaria mellea (inoculated on 18th of September 2014)
GLU2 _ ganoderma lucidum (inoculated on 18th of September 2014)
PCO1 _ pleurotus cornucopie (inoculated on 18th of September 2014)

Armillaria Mellea after 6 days, 9 days and 21 days:

ARM_t1 ARM_t2 ARM_t3

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in vitro mycoTests

Grazie alla disponibilità offertami dal Centro di Micologia Applicata della facoltà di Agraria dell’Università di Bologna, ho eseguito alcuni test con lo scopo di iniziare ad indagare la possibilità di guidare e indirizzare la crescita del micelio.
Ringrazio per il prezioso contributo la Dott.sa Federica Piattoni, la Prof.sa Alessandra Zambonelli e i Dott. Mirco Iotti e Dott.sa Pamela Leonardi per i consigli.

..
The mycology centre of the Agriculture Faculty at the University of Bologna offered me the possibility to work at their Lab and make some in vitro tests with mycelia. The purpose is trying to guide and lead the mycelium growth in a controlled manner.
Thanks to Dott.sa Federica Piattoni, Prof.sa Alessandra Zambonelli, Dott. Mirco Iotti and Dott.sa Pamela Leonardi for their help and suggestions.

glucose handmade pattern

Un primo set di capsule petri è stato preparato lavorando su A-A (agar agar grade a) a diverse concetrazioni di zucchero (glucosio).
140804_gradienteManuale Continue reading